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HPL Oyster Hatchery

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The oyster hatchery at Horn Point is part of a major cooperative research and education effort by the University of Maryland Center for Environmental Science and the Sea Grant Extension Program.

     The program revolves around the original 5,500 ft2 Aquaculture Research Laboratory and the network of agents and specialists that work with the Sea Grant Extension Program. This program, designed to bring together shellfish specialists, hatchery biologists, and educators with oyster growers, students, and other concerned citizens has been in operation since the mid 1970s. A variety of changes in the size and scope of the program as well as changes and improvements in facilities have occurred over time.
Recently, emphasis on the plight of the oyster resources in Chesapeake Bay and elsewhere have brought about an increase in interest in oyster hatcheries as they are beginning to be viewed as viable resources in the fight to bring about major oyster restoration projects in the Upper Chesapeake Bay. Working with outside groups and agencies, the hatchery at Horn Point has seen its production of eyed larvae and spat on shell increase each year beginning with
1994. Currently this facility produces in excess of one to two billion eyed-larvae and over 50 million spat on shell for use in its program.
     The Shellfish section of this facility is comprised of three major components, each dealing with a major area of emphasis for oyster production. The production of cultured algae for use in feeding larval oysters and newly settled spat is accomplished through the use of a series of culture steps, ending with large open vats capable of producing several thousand liters of cultured algae each day.

Algal Carboys
Greenhouse addition to Hatchery
Mass algae culture tanks
in greenhouse

     Broodstock are conditioned in a flow-thru system using water pumped from the Choptank River. River water used in conditioning is tempered using a system of plate heat exchangers and then mixed with unfiltered ambient river water and distributed to the broodstock conditioning system where it is allowed to pass over the conditioning oysters. In this way oysters are fed using the natural algal cells found in the Choptank River water without supplemental algal feeds. If needed, cultured algae can be introduced into this system to provide additional nutrition to broodstock if natural populations of phytoplankton are too low. Oysters placed in this system will go from "winter" condition, where they contain no visible gonadal systems to "spawning" condition in six to eight weeks.
     Once broodstock ripen and are in full spawning condition, they are removed from the conditioning system and placed on the spawning table. Using a combination of warm water and gonadal products, spawning is induced. In general, ripe broodstock will spawn in two hours or less in this system. Problems with low salinity, disease, or other water quality associated problems may delay or inhibit this activity.

Spawning Table with pans to collect male and female gametes.

     Eggs are counted and sperm is added to insure fertilization and the newly fertilized embryos are distributed into large larval tanks filled with filtered Choptank River water at 26oC to 30oC. where they are allowed to develop and grow. Fed daily with cultured algae normal larval periods range from 11 to 20 days depending upon conditions.In general the majority of the larvae produced from this facility are produced using a "mass spawning" technique where a large number of broodstock are placed into a compartment on the spawning table and allowed to spawn together. Once spawning begins males and females which spawn very differently are separated by sex and allowed to spawn to completion in plastic tubs. During this period larval oysters go through several changes from embryo to trochophore to straight hinge, to umbo, to pediveliger (or eyed) stages. Once pediveliger or eyes stage has been reached the oysters are ready to stop swimming and settle to the bottom to look for an appropriate substrate for them to attach to. This process is called settlement or setting and in reality is a metamorphosis for the oyster and is very stressful. Mortalities are often severe during settlement sometimes exceeding 90%.

     At the Horn Point hatchery most eyed larvae are used to produce spat on shell for use in various outplanting experiments or demonstrations. This simple process begins with the production of large amounts of substrate for the oysters to set upon. In the Horn Point hatchery, like most other production setting systems that produce spat on shell, clean aged oyster shell is loaded into plastic mesh bagging material for ease of handling. These bags are then placed in large setting tanks which are then filled with warm river water. Eyed oyster larvae are added along with algal food and the tank is aerated to provide adequate distribution of the larvae throughout the tank.

Piles of shellbags ready for use in setting tanks.
Students loading shellbags into setting tanks.

Within 48 hours the larvae should have completed metamorphosis and settled onto the shells in the tanks. At this point ambient river water is introduced into the tank to provide food for the newly settled oysters. Oysters are now known as spat and quickly grow on the shells. Three to seven days later they have grown enough to be handled and are ready to be transported to the nursery where further growth and hardening will take place.
In the Chesapeake Bay the nursery process takes four to eight weeks. During this time the spat will go from an almost invisible size of under 1 mm to over 15 mm. At this point they are ready to withstand the rigors of movement and are large enough to survive being planted on the bottom for growout.

     Survival in the nursery phase of the operation has been excellent at Horn Point sites approximating 70%. To date over 100 acres of oyster beds have been planted using oysters produced from the Horn Point hatchery. Growth and survival of these spat has exceeded expectations and many have reached market size. Plans are to greatly expand efforts using hatcheries as tools for oyster rehabilitation in Maryland.
     The addition of a 2,400 ft2 greenhouse to the hatchery in 1998 has greatly expanded the capacity of the Horn Point facility. This new greenhouse along with a greatly improved setting and cultch handling system is capable of producing over 4 million spat on shell per week during the peak setting season. With this expanded facility came the need for greater manpower, more funding and a host of other logistical problems. These have been solved by establishing a cooperative program that incorporates support from a host of other groups and individuals.
Spat on shell produced at HPL.

Personnel:Dr. Donald W. Meritt (Program Director) meritt@hpl.umces.edu

Stephanie Tobash (Hatchery Manager) tobash@hpl.umces.edu

Angela Padeletti (Hatchery Technician) apadelet@hpl.umces.edu

Melissa Radcliffe (Algologist) mwood@hpl.umces.edu

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